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IRF4 Recombinant Rabbit mAb (bsm-62837R)  
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產(chǎn)品編號(hào) bsm-62837R
英文名稱 IRF4 Recombinant Rabbit mAb
中文名稱 干擾素調(diào)節(jié)因子4重組兔單抗
別    名 LSIRF; Multiple myeloma oncogene 1; NF-EM5; IRF4; MUM1; Transcriptional activator PIP.  
抗體來源 Rabbit
克隆類型 Recombinant
克 隆 號(hào) 4G6
交叉反應(yīng) Human
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1:50-100,ICC/IF=1:50-200,IP=1:20-50
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 52 kDa
檢測(cè)分子量 52
性    狀 Liquid
免 疫 原 A synthesized peptide derived from human IRF4: 144-450/451 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
保存條件 Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Transcriptional activator. Binds to the interferon-stimulated response element (ISRE) of the MHC class I promoter. Binds the immunoglobulin lambda light chain enhancer, together with PU.1. Probably plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells.

SWISS:
Q15306

Gene ID:
3662

轉(zhuǎn)錄調(diào)節(jié)因子(Transcriptin Regulators) 干擾素調(diào)節(jié)因子家族是一大類對(duì)干擾素起調(diào)控作用的轉(zhuǎn)錄因子的統(tǒng)稱。一般認(rèn)為干擾素調(diào)節(jié)因子(IRF)通過調(diào)節(jié)干擾素的表達(dá)而行使其抗病毒、應(yīng)激、免疫調(diào)節(jié)功能。近年來,研究人員發(fā)現(xiàn)IRF在細(xì)胞凋亡、細(xì)胞周期、細(xì)胞分化、腫瘤發(fā)生中也起著重要的調(diào)節(jié)作用。
產(chǎn)品圖片
25 ug total protein per lane of various lysates (see on figure) probed with IRF4 monoclonal antibody, unconjugated (bsm-62837R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with IRF4 Monoclonal Antibody, Unconjugated(bsm-62837R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with IRF4 Monoclonal Antibody, Unconjugated(bsm-62837R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with IRF4 Monoclonal Antibody, Unconjugated(bsm-62837R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with IRF4 Monoclonal Antibody, Unconjugated(bsm-62837R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
The KARPAS-299 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Rabbit Anti-IRF4 antibody (bsm-62837R,1:100); Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488 (bs-60295G-BF488): 1 μg/test. Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
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