| 產(chǎn)品編號 | bsm-62863R |
| 英文名稱 | Phospho-MAP2 (Ser136) Recombinant Rabbit mAb |
| 中文名稱 | 磷酸化微管相關(guān)蛋白2重組兔單抗 |
| 別 名 | MAP 2; MAP dendrite specific; MAP2; MAP2A; MAP2B; MAP2C; Microtubule associated protein 2; Microtubule-associated protein 2. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 19F17 |
| 交叉反應 | Human,Mouse,Rat |
| 產(chǎn)品應用 | WB=1:1000-1:2000,IHC-P=1:100-1:200,IHC-F=1:100-1:200,IF=1:50-1:200,ICC/IF=1:50-1:200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 200 kDa |
| 檢測分子量 | 70280 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human MAP2 around the phosphorylation site of S136: NLPP-pS-PPP(全長1827aa) |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules. SWISS: P11137 Gene ID: 4133 微管相關(guān)蛋白 2（MAP-2)是一種磷蛋白質(zhì)，在正常腦組織中，主要存在于神經(jīng)元的胞體、樹突和樹突棘，是腦內(nèi)最豐富的蛋白之一。MAP-2在其調(diào)節(jié)微管的聚合作用和微管的穩(wěn)定性以及對神經(jīng)元軸突和樹突的發(fā)生、延長、穩(wěn)定和突觸可塑性調(diào)節(jié)具有重要作用。 |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded Human colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-MAP2 (Ser136) Monoclonal Antibody, Unconjugated (bsm-62863R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-MAP2 (Ser136) Monoclonal Antibody, Unconjugated (bsm-62863R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-MAP2 (Ser136) Monoclonal Antibody, Unconjugated (bsm-62863R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-MAP2 (Ser136) Monoclonal Antibody, Unconjugated (bsm-62863R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-MAP2 (Ser136) Monoclonal Antibody, Unconjugated (bsm-62863R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-MAP2 (Ser136) Monoclonal Antibody, Unconjugated (bsm-62863R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
