| 產(chǎn)品編號 | bsm-62921R |
| 英文名稱 | Ras Recombinant Rabbit mAb |
| 中文名稱 | 原癌基因Ras重組兔單抗 |
| 別 名 | GTPase HRas; GTPase KRas; GTPase NRas; H-Ras-1; H-RASIDX; Ha-Ras; HAMSV; HRAS; K RAS2A; K RAS2B; K RAS4A; K RAS4B; K-RAS; KRAS; N-RAS; N-terminally processed; NRAS; p21ras; RASH1; RASK2. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200,Flow-Cyt=1:20-50,ICC/IF=1:50-200,IP=1:20-50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 21,22 kDa |
| 檢測分子量 | 22 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human Ras: 25-50 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. SWISS: P01111 Gene ID: 4893 細胞信號轉(zhuǎn)導(dǎo)系統(tǒng)紊亂是腫瘤細胞生長的重要特征之一，Ras蛋白參與體內(nèi)多種細胞信號轉(zhuǎn)導(dǎo)途徑而發(fā)揮作用,而原癌基因N-ras是一種多功能的細胞因子，廣泛存在于自然界，N-ras在多種細胞生命活動中起極為重要的作用，包括細胞的增殖、分化和細胞骨架的構(gòu)建等等，該抗體主要用于腫瘤方面的研究。 |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with Ras monoclonal antibody, unconjugated (bsm-62921R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
