| 產(chǎn)品編號 | bsm-62927R |
| 英文名稱 | Phospho-PAK1/2/3 (Ser144/Ser141/Ser154) Recombinant Rabbit mAb |
| 中文名稱 | 磷酸化p21激活激酶1/2/3重組兔單抗 |
| 別 名 | ADRB2; alpha-PAK; CDC42/RAC effector kinase PAK-A; EC 2.7.11.1; P65-PAK; P68-PAK; PAK1(phospho S144); PAK2(phospho S141); PAK3(phospho S139). |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 9G6 |
| 交叉反應 | Human,Mouse,Rat |
| 產(chǎn)品應用 | WB=1:500-1:5000,IHC-P=1:100-1:200,IHC-F=1:100-1:200,IF=1:100-1:200,Flow-Cyt=1:20-1:100,ICC/IF=1:50-1:200,IP=1:20-1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 58-62 kDa |
| 檢測分子量 | 65 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human PAK1 around the phosphorylation site of S144: YM-pS-FT |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, or cell cycle regulation. SWISS: Q13153 Gene ID: 5058 PAK1-3蛋白具有廣泛的生物學功能,也是一個保守的絲氨酸/蘇氨酸蛋白激酶,參與許多重要的細胞活動。包括細胞骨架的動力學調節(jié),細胞移動,生存和凋亡,細胞周期,基因轉錄調節(jié),細胞生長信號轉導和轉化等。 |
| 產(chǎn)品圖片 |
MCF-7 (H) cells were treated with or without EGF (1μg/ml) for 10 min, 25 μg total protein per lane of cell lysates (see on figure) probed with Phospho-PAK1/2/3 (Ser144/Ser141/Ser154) monoclonal antibody, unconjugated (bsm-62927R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-PAK1/2/3 (Ser144/Ser141/Ser154)? Monoclonal Antibody, Unconjugated(bsm-62927R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-PAK1/2/3 (Ser144/Ser141/Ser154)? Monoclonal Antibody, Unconjugated(bsm-62927R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-PAK1/2/3 (Ser144/Ser141/Ser154)? Monoclonal Antibody, Unconjugated(bsm-62927R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-PAK1/2/3 (Ser144/Ser141/Ser154)? Monoclonal Antibody, Unconjugated(bsm-62927R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
