| 產(chǎn)品編號(hào) | bsm-62929R |
| 英文名稱 | PAK1 Recombinant Rabbit mAb |
| 中文名稱 | p21激活激酶1重組兔單抗 |
| 別 名 | ADRB2; p21 activated kinase 1; p65 PAK; PAK alpha; Pak1; Paka; PAKalpha; Protein kinase MUK2. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號(hào) | 14G3 |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1:20-50,ICC/IF=1:50-200,IP=1:20-50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 61 kDa |
| 檢測(cè)分子量 | 66 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human PAK1: 45676 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Protein kinase involved in intracellular signaling pathways downstream of integrins and receptor-type kinases that plays an important role in cytoskeleton dynamics, in cell adhesion, migration, proliferation, apoptosis, mitosis, and in vesicle-mediated transport processes. SWISS: Q13153 Gene ID: 5058 PAK1-3蛋白具有廣泛的生物學(xué)功能,也是一個(gè)保守的絲氨酸/蘇氨酸蛋白激酶,參與許多重要的細(xì)胞活動(dòng)。包括細(xì)胞骨架的動(dòng)力學(xué)調(diào)節(jié),細(xì)胞移動(dòng),生存和凋亡,細(xì)胞周期,基因轉(zhuǎn)錄調(diào)節(jié),細(xì)胞生長(zhǎng)信號(hào)轉(zhuǎn)導(dǎo)和轉(zhuǎn)化等。 |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with PAK1 monoclonal antibody, unconjugated (bsm-62929R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PAK1 Monoclonal Antibody, Unconjugated(bsm-62929R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PAK1 Monoclonal Antibody, Unconjugated(bsm-62929R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PAK1 Monoclonal Antibody, Unconjugated(bsm-62929R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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