| 產品編號 | bsm-62997R |
| 英文名稱 | TRAP1/HSP75 Recombinant Rabbit mAb |
| 中文名稱 | 熱休克蛋白75重組兔單抗 |
| 別 名 | HSP75; HSP 75; HSP90L; TRAP-1. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 4C3 |
| 交叉反應 | Human |
| 產品應用 | WB=1:1000-1:2000,IP=1:20-1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 80 kDa |
| 檢測分子量 | 75 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human Hsp75: 630-680 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Chaperone that expresses an ATPase activity. Involved in maintaining mitochondrial function and polarization, downstream of PINK1 and mitochondrial complex I. Is a negative regulator of mitochondrial respiration able to modulate the balance between oxidative phosphorylation and aerobic glycolysis. The impact of TRAP1 on mitochondrial respiration is probably mediated by modulation of mitochondrial SRC and inhibition of SDHA. SWISS: Q12931 Gene ID: 10131 信號傳導（Signaling Intermediates） HSP75/TRAP-1與TNF受體發(fā)生相互作用,可產生促進TNF的信號轉導。 |
| 產品圖片 |
Western blot analysis of K562 cell lysate. Using TRAP1/HSP75 (bsm-62997R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
