| 產品編號 | bs-3386R |
| 英文名稱 | Phospho-NR1D1 (Ser55 + Ser59) Rabbit pAb |
| 中文名稱 | 磷酸化細胞核受體Rev-Erbα抗體 |
| 別 名 | NR1D1(phospho Ser55+Ser59); NR1D1(phospho S55+S59); EAR-1; EAR1; ERBA-RELATED 1; hRev; Nr1d1; NR1D1_HUMAN; Nuclear Receptor Rev-ErbA alpha; Nuclear receptor subfamily 1 group D member 1; Orphan nuclear receptor NR1D1; Rev erbAalpha; Rev erbalpha; Rev-erbA-alpha; Rev-ErbAalpha; REV-ERBalpha; THRA1; THRAL; Thyroid hormone receptor alpha-like; Thyroid hormone receptor, alpha like; V-erbA related protein EAR-1; V-erbA-related protein 1. |
| 產品類型 | 磷酸化抗體 |
| 研究領域 | 細胞生物 免疫學 染色質和核信號 神經生物學 信號轉導 生長因子和激素 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human (predicted: Mouse,Rat,Sheep,Cow) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 67 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human REV-ERB alpha around the phosphorylation site of Ser55/59: PP(p-S)PTG(p-S)LT |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
NR1D1, a NR1 Thyroid Hormone-Like Receptor, is encoded by the same genomic locus as, but transcribed from the opposite strand of, Thyroid Hormone Receptor Alpha (TR Alpha). NR1D1 is a target of Nuclear Receptor ROR Alpha and a transcription regulator that has been shown to affect myocyte differentiation, adipogenesis, and lipoprotein metabolism. Mice lacking NR1D1 show abnormal postnatal cerebellar development. NR1D1 expression has been documented in human skeletal muscle and a variety of mouse and rat tissues. ESTs have been isolated from human tissue libraries, including cancerous adrenal, blood, brain, breast, colon, duodenum, fetus, head/neck, kidney, lung, skeletal muscle, skin, synovium, uterus, normal brain, breast, colon, eye, heart, pancreas, pituitary, prostate, skeletal muscle, skin, testis and thyroid. Function: Functions as a constitutive transcriptional repressor. In collaboration with SP1, activates GJA1 transcription. Possible receptor for triiodothyronine. Subunit: Interacts with C1D and NR2E3. Interacts with SP1. Subcellular Location: Nucleus (Potential). Tissue Specificity: Expressed in all tissues and cell lines examined. Expressed at high levels in some squamous carcinoma cell lines. Similarity: Belongs to the nuclear hormone receptor family. NR1 subfamily. Contains 1 nuclear receptor DNA-binding domain. Gene ID: 9572 Database links: Entrez Gene: 9572 Human Entrez Gene: 217166 Mouse Omim: 602408 Human SwissProt: P20393 Human SwissProt: Q3UV55 Mouse Unigene: 592130 Human Unigene: 724 Human Unigene: 390397 Mouse Unigene: 29848 Rat 1.細胞核受體Rev-Erbα是生物鐘系統(tǒng)的重要組成部分,不僅是生物鐘基因而且是生物鐘調節(jié)基因,在維持生物節(jié)律準確性中發(fā)揮著重要作用； 2.Rev-Erbα調節(jié)糖代謝、脂質代謝、脂肪形成、纖溶蛋白降解、血管炎癥并與其他能量平衡相關核受體相互作用。 |
| 產品圖片 |
Tissue/cell: human laryngeal tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Phospho-NR1D1 (Ser55 + Ser59) Polyclonal Antibody, Unconjugated(bs-3386R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human laryngeal tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Phospho-NR1D1 (Ser55 + Ser59) Polyclonal Antibody, Unconjugated(bs-3386R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control（black line）:Hela.
Primary Antibody (green line): Rabbit Anti-Phospho-NR1D1 (Ser55 + Ser59) antibody (bs-3386R)
Dilution:1ug/Test;
Secondary Antibody（white blue line）: Goat anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Isotype control（orange line）: Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
