| 產(chǎn)品編號 | bsm-60269R |
| 英文名稱 | BOB-1 Recombinant Rabbit mAb |
| 中文名稱 | B細胞特異性轉錄因子OBF-1重組兔單抗 |
| 別 名 | B-cell oct-binding protein 1; B-cell-specific coactivator OBF-1; POU domain class 2-associating factor 1; POU2AF1; OCT-binding factor 1; BOB-1; OCA-B. OBF1; OCA-B; OBF1_HUMAN; BOB.1. POU domain class 2-associating factor 1; POU2AF1 (BOB1, OBF1) |
| 研究領域 | 轉錄調節(jié)因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | B12H9 |
| 交叉反應 | Human |
| 產(chǎn)品應用 | WB=1:500-1000,IHC-P=1:200-1:1000,IHC-F=1:50-100,IF=1:50-100,ICC/IF=1:50-100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 28 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human BOB-1 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Enables transcription coactivator activity. Involved in positive regulation of transcription by RNA polymerase II. Part of RNA polymerase II transcription regulator complex. [provided by Alliance of Genome Resources, Nov 2021] SWISS: Q16633 Gene ID: 5450 |
| 產(chǎn)品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Lysate: Ramos, Daudi, Raji
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted MW: 27 kDa
Observed MW: 35 kDa
Tissue: Human lymphoma
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000
Primary ab incubation condition: 1 hour at room temperature
Counter stain: Hematoxylin
Comment: Color brown is the positive signal for bsm-60269R
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
