| 產品編號 | BIO0950SM |
| 英文名稱 | Anti-EGFR & Fc-gamma-RIIIA Reference Antibody (AFM24 Biosimilar) |
| 中文名稱 | |
| 別 名 | ERBB1 / EGFR / HER1 & FcgR3a / CD16a; AFM24 |
| 抗體來源 | |
| 克隆類型 | Monoclonal |
| 克 隆 號 | |
| 交叉反應 | Human |
| 產品應用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 74.5 kDa |
| 檢測分子量 | |
| 性 狀 | Lyophilized |
| 亞 型 | IgG-ScFv |
| 純化方法 | Protein A |
| 緩 沖 液 | 100 mM Pro-Ac 20mM Arg pH 5.0 |
| 保存條件 | -80°C for 2 years under sterile conditions； -20°C for 1 year under sterile conditions；Avoid repeated freeze-thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 | |
| 產品圖片 |
Afm24 bound to EGFR-CHO-K cells, and then rebounded to fluorescent secondary antibodies (Anti-human IgG, Fcγ PE) , and test by flow cytometry. As shown in fig, Afm24 bound to EGFR-CHO-K cells, and the EC50 was 4.075 nM.
Afm24 bound to CD16a protein, and then rebounded to secondary antibodies (Anti-human-IgG-Fc-HRP) , and read OD450. As shown in fig, Afm24 bound hu-CD16a-His, and the EC50 was 0.489 nM.
Afm24 bound to EGFR protein, and then rebounded to secondary antibodies (Anti-human-IgG-Fc-HRP) , and read OD450. As shown in fig, Afm24 bound hu-EGFR-His, and the EC50 was 0.044 nM.
The purity of Anti-EGFR & Fc-gamma-RIIIA Reference Antibody (AFM24) is 97.37%, determined by SEC-HPLC.
Anti-EGFR & Fc-gamma-RIIIA Reference Antibody (AFM24) on SDS-PAGE under reducing (R) condition. The purity of the protein is greater than 99.5%.
The detected molecular weight of Anti-EGFR & CD16a/Fc-gamma-RIIIA Reference Antibody (Afm24) is 196.31 kDa.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
